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Hematocrit, reticulocyte count, vitamin B12, folate and iron levels should be obtained prior to treatment

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It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile

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A reticulocyte count may also be checked, as a rise in reticulocytes (young red blood cells) within the first week indicates effective bone marrow response

While oral B12 is widely available, its not always the most effective, especially for individuals with absorption issues (like those with digestive disorders or older adults)