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The acetyl group from acetyl-l-carnitine is responsible for production of acetylcholine, an important neurotransmitter for optimal mental functioning, memory and attention

The complex is then adsorbed to calmodulin Sepharose beads (via the CBP tag) and eluted with EGTA

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The protein-bound dye was dissolved in 10 mM Tris base solution (ThermoFisher, 15504-020) to obtain a reading at 510 nm

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