After deep anaesthesia with isoflurane (Attane Vet, 1,000 mg g 1 , ScanVet, Piramal Critical Care) mice were decapitated and the brain was removed and submerged in ice-cold aCSF (124 mM NaCl, 5 mM KCl, 1.2 mM Na 2 HPO 4 2H 2 O, 2.7 mM CaCl 2 2H 2 O, 1.2 mM MgSO 4 (anhydrous)), 10 mM dextrose, 26 mM NaHCO 3 was adjusted to pH 7.4 and an osmolarity of 298302 mOsm kg 1 after saturation with carbogen (95% O 2 /5% CO 2 )
Microdosing is typically taken once weekly
To decrease appetite and mimic the effects of the medication, include at least 25 grams of protein, 15 grams of fiber, less than 45 grams of carbohydrates and no more than 10 grams of added sugar and no more than 10 grams of fat in your first meal of the day
Add heart-healthy nutrients to your daily routine, including Omega-3 fatty acids
Further details of the search strategy are shown in Appendixes 1 & 2
Excess caffeine plus reduced fluid intake can cause dehydration and headaches