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By contrast, two other studies showed that TGR5 was not necessary for the benefits of either VSG 73 or RYGB 91
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A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)
PS, parastrial nucleus
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