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& Clayden, J

however, none had hypoglycaemic symptoms, which suggests the test has low specificity 87

The activation of VIP receptors stimulates the adenylyl cyclase to generate cytosolic cAMP that activate the cystic fibrosis transmembrane conductance regulator (CFTR), resulting in secretion of Cl into the lumen

Real-time quantitative polymerase chain reaction amplification was done utilizing PowerUp SYBRTM Green Master Mix (Thermo Fisher Scientific), and data were analyzed with an Applied Biosystems and software version 3.1 (Thermo Fisher Scientific, MA, USA)