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Off-target detection To determine the site-specific cleavage of the CRISPRCas9 system in vivo, the potential off-target loci were searched using Cas-OFFinder ( The selected potential off-target sites were initially PCR-amplified and then subjected to a DNA sequencing assay

Central and peripheral mechanism of MOTS-c attenuates pain hypersensitivity in a mice model of inflammatory pain

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