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Insert the needle at a slight angle through the stopper

Cloudiness can indicate aggregation, contamination, or temperature damage

Direct the water against the inner wall of the vial as it is injected never onto the lyophilized cake, since direct impact foams the solution and denatures compound at the air-water interface

However, as with any medical intervention, some mild side effects can occur, such as slight pain, redness, or swelling at the injection site