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coli , gene synthesized (Biomatik), and inserted into pET28a vector using Eco R1 and Nde 1 sites in frame with an N-terminal 6x-His tag using a previously described cloning method (Schenck et al., 2015)

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B12 deficiency can also cause diarrhea, nausea, constipation, bloating, gas and other gastrointestinal symptoms

If you dont have enough B12, you cant metabolise your methylfolate

To view a copy of this license, visit About this article Cite this article Garvey, W.T., Batterham, R.L., Bhatta, M