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While the emphysema state results in loss of alveolar spaces and low sputum production, the bronchitis phenotype results in narrowing of the airways as well as blocking of the alveolar spaces with mucus obstruction leading to chronic cough, sputum production, and exacerbations (Szalontai et al., 2021

The cells after 3-4 passages were used in the following experiments 20,62

doi:10.1073/pnas.12.2.132