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Conditioned medium was extracted by taking 10 l of the medium supernatant and mixing it with 390 l of the extraction solvent

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The benzyl alcohol preservative stops bacterial growth after the vial is opened

Originally developed as a tanning agent (acting via MC1R receptors), it also binds to melanocortin receptors in the brain (particularly MC3R and MC4R) that influence sexual function

Next, the solution was stirred for 10 min under a temperature of 80 C using a magnetic stirrer