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2 Materials and methods 2.1 The experimental animals Currently, we included 36 adult male albino Wistar rats (with a body mass range equal to 190230 g, 810 weeks of age) supplied by the Faculty of Veterinary Medicine, Zagazig University. Rats were kept in hygienic laboratory conditions, including temperature (22C 3C), relative humidity (60% 10%), and normal light/dark cycle

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10.2 Mechanisms and epidemiology Nociplastic pain stems from central sensitization, involving abnormal pain processing in the peripheral and central nervous systems, leading to increased sensitivity and reduced inhibition

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