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The size of the punch biopsy can vary from 1 mm to 2 mm, but it is preferable to use a punch 1.5 mm or larger

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fumigatus strains ATCC46645 and ATCC26933 were co-transformed with two DNA constructs, each containing an incomplete fragment of a pyrithiamine resistance gene ( ptrA ) [21] fused to 1.2 kb, and 1.3 kb of gliT flanking sequences, respectively