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Regarding the optimal dosing of methylene blue, existing evidence suggests that higher bolus doses of methylene blue (e.g., 4 mg/kg) may provide mortality benefits in patients with septic shock specifically, though both high and low doses reduced vasopressor requirements

Reconstitution math: choosing your concentration The formula is simple: Concentration (mg/ml) = Total peptide (mg) / Volume of water added (ml) So if you have a 30 mg vial and add 3 ml of bacteriostatic water, your concentration is 30/3 = 10 mg/ml