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CHO-K1 (ATCC #CCL-61) cells were maintained in F12 containing 10% (v/v) FBS, 100 units/mL penicillin and 100 mg/mL streptomycin at 37 C in 5% CO 2

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The red blood cells were washed three times with 500 l of 0.9% sodium chloride, after which 500 l of the erythrocyte suspension was combined with 2 ml of ice-cold double-distilled water