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doi: 10.2147/tcrm.2007.3.1.133 73 YangCMLuoSFHsiehHLChiPLLinCCWuCCet al

Samples were harvested after 7- and 14-days incubation and extracted in lysis buffer (50 mM Tris pH7.4, 150 mM NaCl 2 and 0.01% Tween80) by bead-beating with 0.1 mm zirconia-silica beads (Sigma) in the FastPrep at 6.5 for 3 45 s with careful cooling between bursts

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It represents the point at which bacterial inhibition from the benzyl alcohol begins to weaken, and peptide degradation from repeated temperature fluctuations (each time you take the vial out to draw a dose) accumulates to meaningful levels

Storage and formulation quality can dramatically reduce effective half-life before the experiment even begins

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