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Nonetheless, one additional contact was found to be unique for compound 2-bound structure, i.e., a hydrogen bond between the backbone atom of L260 in ICL2 and R38 in the GH5 subunit (Supplementary Fig

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Our probe was specific to gld-1 : transcripts were patterned as described previously in wild type [41, 46] and cytoplasmic gld-1 mRNAs were undetectable in gld-1(q485) , a deletion mutant that likely renders transcripts subject to non-sense mediated decay [45] ( S8 Fig )

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