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Not all the screen phenotypes could be confirmed in agar-overlay plaque assays, possibly due to variations in cell growth between liquid and solid media, or due to the requirement for successive complete infection cycles and sufficient burst sizes for the formation of visible plaques that would render plaque assays less sensitive than single-infection cycle dynamics in our screen

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Additionally, the NNMT substrate nicotinamide 12 is metabolized by the microbiome 56,57 , and thus NNMTi may modulate substrate availability, potentially influencing microbial metabolic capacity to drive the NNMTi-mediated weight loss