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An estimated 15 g whole cell extract was loaded per well on 10%-12% SDS-PAGE gel and transferred onto nitrocellulose membrane (Bio-Rad)

Neurological Function Score (Modified Longa System) Neurological deficits were evaluated 1 hour after ET-1 injection using the modified Longa scoring system: 0: No observable neurological deficit (excluded from analysis) 1: Failure to fully extend the left forepaw 2: Consistent circling to the left 3: Falling or leaning to the left side when ambulating 4: No spontaneous movement (severe infarction) Animals scoring between 1 and 3 were considered to have moderate ischemic deficits and were included in further analyses

Following oral ingestion, rapid elevation in plasma concentrations of both d 9 -BB and d 9 -l-carnitine was observed, followed by d 9 -TMAO generation after a lag phase (Figure 2, AC, filled circles)

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The same studies can be located independently on PubMed using the CAS number (13441-78-4) or the compound name

10.1007/s40262-022-01147-w 15 ChalmersJ