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However, C14, C14OH, and C18OH acylcarnitine levels remained almost unaltered in the patients and healthy control groups

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The cell lysates were solubilized in RIPA buffer containing 1 mM sodium orthovanadate, 1 mM sodium fluoride, 10 mM N-ethylmaleimide, 5 g/mL leupeptin, 5 g/mL aprotinin, and 2 mM phenylmethylsulphonyl fluoride, after which they were immunoprecipitated with FLAG beads

Because of its gastrointestinal side effects, Metformin should be started at a low dose with gradual titration, depending on tolerance