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After fixation, coverslips were washed 3 times with TBST then blocked with 10% goat serum, 1% BSA, 0.1% gelatin, 22.52 mg/mL glycine, and 0.1% Triton X-100 in TBST for 30 minutes

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In lung tissue homogenates, Gpx3, but not Sod3, was upregulated and detergent solubility profiling indicated that Gpx3 associated with ECM proteins