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After glucose depletion in YP20D medium, a feeding solution containing 200 g/L yeast extract, 100 g/L peptone, and 600 g/L glucose was continuously supplied

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This activation is associated with elevated mitochondrial reactive oxygen species (mtROS), which can trigger the mitochondrial localization and pore-forming activity of GSDMD, thereby facilitating a transition from apoptotic to necroptotic cell death