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Subsequently, proteins were desalted and incubated in the presence of either DTT, GSH or GSH in combination with NADPH and GR

This energy exhaustion will further lead to the production of reactive oxygen species (ROS), apoptosis, and abnormal calcium homeostasis, which will promote epilepsy, thus forming a vicious circle (5)

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consequently, five forms of phosphofructokinase can be identified that differ in subunit composition: M 4 , M 3 L 1 , M 2 L 2 , ML 3 , and L 4