Pickart and Margolina (2018) used large geneexpression databases to examine how GHKCu affects human cells and found that it can alter the activity of more than 4,000 genes involved in tissue repair, antiinflammatory pathways, and antioxidant defenses [8]
4 Materials and methods 4.1 Cell culture and in vivo experiments 4.1.1 Cell lines Four human NB cell lines were used in this paper: Be(2)-C (MNA), IMR5-/75-shMYCN (29) (MNA, doxycycline-inducible shRNA targeting MYCN, resulting in MYCN downregulation), IMR-32 (MNA), and SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (non-MNA, doxycycline-inducible transgene, resulting in MYCN overexpression)
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