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In each well containing 0.8 mL of phosphate buffer solution (0.1 M, pH 7.4), 0.1 mL of NAD + (nicotinamide adenine dinucleotide, oxidized form 10 mM), 0.05 mL of sample, and finally glutamate dehydrogenase enzyme (0.05 mL) were added successively

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PMID 30734066

Changes in pigment lesions , moles (nevi) enlarged and darkened , some patients developed new ones