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The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

But although reperfusion treatment is absolutely necessary for tissue survival, it also contributes to additional tissue damage (47, 78) that can cause HT, which will lead to a worse clinical outcome and patient recovery

Viruses were divided into aliquots and stored at 80C before their use

& McCarty, M

The relative likelihood of iron overload versus other causes of organ dysfunction depends on a number of factors that affect the likelihood of possible diagnoses, including patient age, family history, other symptoms and other comorbidities