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Storing them in plastic syringes for extended periods can increase the risk of degradation and adsorption to the plastic

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The thermal cycling comprised an initial denaturation step at 95 C for 4 min, followed by 35 cycles at 95 C for 30 s, 59 C for 30 s and 72 C for 30 s, with the final extension at 72 C for 7 min

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19881993: Maquart et al

This could explain why some users report such dramatic improvements even in chronic injuries that had been resistant to other treatments