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These symptoms usually improve over time

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Washed beads were eluted in 20 l 0.2% SDS in 50 mM HEPES with 4 Laemmli sample buffer (Bio-Rad, 1610747) and boiled at 95 C for western blot analysis

The accumulation of D-Carnitine over time could be a result of minor degradation or evaporation of the solvents (diluent) since they were sensitive to changes in stability

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