Jacques, F., Rippa, S
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Li Y, Miao LY, Xiao YL, Huang M, Yu M, Meng K, et al
Using 5-chloromethylfluorescein diacetate (CMFDA), which has been reported to be a potent and irreversible GSTO1 inhibitor 22 , we optimized a gel-based binding assay that measures competitive inhibition of CMFDA binding to recombinant GSTO1 or endogenous GSTO1 in a soluble proteome (Supplementary Fig
Avoid freezing reconstituted solution as this damages peptide structure
2 weeks before basketing, 5 consecutive days
