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2.5 Affinity isolation of HPX binding proteins We used well-controlled affinity chromatography to isolate putative hemopexin receptors (HPXRs) from whole cell extracts (100,000 x g isolates in HEPES buffered pH 7.4 Triton X-100 0.5%, 150 mM NaCl containing phosphatase and protease inhibitors, Millipore Sigma) of non-hepatic human promyelocytic HL-60, HepG2 cells, and freshly plated primary human hepatocytes

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If it is consistently dark despite drinking adequate fluids, you likely need more electrolytes, not more water

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