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Development of high-throughput (next-generation) sequencing allowed DNA methylome analysis to be more sensitive and less biased than approaches based on enzymatic digestion or methylated DNA immunoprecipitation combined with hybridization to microarrays (for review, see Lister and Ecker (2009))

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DOI: 10.1111/tid.12353 40

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3 mL) determination (VARIANT II TURBO Hb Testing System, Bio-Rad, Hercules, CA, USA)