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Specifically, 2 mM FeSO 4 solution, 360 mg mL 1 safranine solution, 3% (wt%) H 2 O 2 solution, 0.1 M HCl (aq) and G/D-CuP were added to each well in a 96-well plate (n = 3)
Whilst we found that ex vivo vehicle treated cells produced high levels of cytokines, including IL-5, IL-13, IL-9 ( Supplementary Figures 4BD ), and modest amounts of IL-10 and IL-6 ( Supplementary Figures 4E, F ), melatonin treatment greatly reduced the concentration of all cytokines analyzed ( Supplementary Figures 4B F )
These effects of nicotinic acid are exerted by it binding to and activating the hydroxycarboxylic acid receptor 2 (HCA 2 ) which was originally identified as GPR109A
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