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Our finding that sleep loss does not alter the amount of circulating GLP-1 in male subjects corroborates recent observations of reduced afternoon GLP-1 concentrations in women but not in men after sleep curtailment
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Welcome to the PlexusDx Education Hub, where we unpack GLP-1 therapies, weight-management protocols, and the genetic factors sitting underneath every metabolic decision
The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C
The GWAS was performed including covariates as described in equation 1 above, with the addition of five genetic principal components to account for fine-scale genetic ancestry, and indicator variables to account for variation in the genotyping platform
JAMA Neurol 2019;76:420429.ArticlePubMedPMC 114
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