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By inhibiting the pathways responsible for muscle protein breakdown, HMB helps women preserve metabolically active lean tissue during the exact periods when it's most vulnerable to loss

RNA isolation and qRTPCR analysis Total RNA was extracted using TRIzol (Takara Bio, Tokyo, Japan) and quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed using Hieff qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) according to the manufacturers instructions

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Drissi H, Lieberherr M, Hott M, Marie PJ, Lasmoles F

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