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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s
Similarly, gene-editing technologies like CRISPR offer potential breakthroughs but come with ethical dilemmas and safety concerns Doudna & Charpentier, Science , 2014
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