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Subsequently, the enzyme solution was mixed with 6X MAT buffer pH 10.28 (volume ratio 1:2) to increase the pH of the solution and induce the formation of the thiolate-FAD charge transfer

Table 1 below presents a listing of compounds in Family 1 , drawn to mimetics, and Famillies 2-5, drawn to antagonists, all of which have been synthesized and analyzed according to the procedures described above

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