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In conclusion, and in line with its 2 h half-life in patients, in vitro studies should be carried out thoroughly considering ascorbic acid chemistry with recommended experimental conditions such as avoiding catalytic metals in culture media, using a dosing per cell metric scheme and a 2 h treatment with a media refresh step [13, 126, 156]

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Future work will use longitudinal profiling and single-cell sequencing to assess cell-type-specific GSH roles 5 Conclusion In conclusion, by leveraging multi-omics integration, the present study identified GSH metabolism as a pivotal node in SAE, while providing a comprehensive molecular framework for SAE pathogenesis, and linking microglia-driven neuroinflammation to hippocampal oxidative damage

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