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Add trypsin antigen retrieval solution and incubate at 37 C for 15 min

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Based on these observations with GEP12, and on our unexpected findings that GEP44-mediated increases in ISR and decreases in glucagon release can be detected only in the presence of Y1-R antagonists, we proceeded to characterize the contributions of Y1-R to glucose uptake in muscle tissue

doi: 10.1530/JOE-19-0399