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Material and methods 2.1 Animals and treatments All animal procedures have been approved by a local animal care and use committee (CECCAPP) and subsequently authorized by the French Ministry of Research (APAFIS #40481-2022103017295302 v3)
Proline, 4-hydroxyproline, and their small peptides are transported in blood in the free form
If nausea persists for more than two weeks at the same dose, that dose is your current ceiling
1 Remove AOD-9604 vial from refrigeration and allow to reach room temperature (1520 minutes) 2 Clean vial top with alcohol swab and allow to air dry completely 3 Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water) 4 Inject BAC water slowly down the side of the vial do not aim directly at the powder 5 Gently swirl in circular motions DO NOT shake vigorously as this degrades the peptide 6 Allow 23 minutes for full dissolution solution should be clear and colorless 7 Draw calculated dose into an insulin syringe for subcutaneous injection 8 Inject into abdomen, thigh, or upper arm
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