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The significance of this N-terminal helix was further demonstrated by deliberately distorting the helical structure by forming a smaller size ring via a disulfide bridge between Cys 16,20 , leading to a substantial loss in receptor interaction

Glutathione (L--glutamyl-L-cysteinyl-glycine) is the most abundant non-protein thiol in mammalian cells, present in millimolar concentrations throughout the body with particularly high concentrations in the liver, kidneys, and immune tissues

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Although this region is not completely conserved in GIPR, at least two residues (R411 and L412) are conserved

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