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Nuclei were isolated using the 10X Genomics Chromium Nuclei Isolation kit including RNase Inhibitor (10X Genomics, PN-1000494), and using the 10X Genomics protocol for Single Cell Multiome ATAC + Gene Expression (10X Genomics, CG000505 Rev A)

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Despite the rapid turnover rates for most cell types present in the intestinal epithelium, Paneth cells have a life span of up to two months and play an important role in the defence against harmful microbes (Mu et al