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This degradation, in turn, allows NF-B activity to increase, resulting in increased transcription of pro-inflammatory genes, including TNF-, IL-1, IL-6, and inducible nitric oxide synthase (iNOS) ( In addition to the NF-B pathway, the mitogen-activated protein kinase (MAPK) signaling cascade is also an important regulator of inflammatory responses in CNS microglia

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Next, glycine is added to \(\gamma\)-glutamylcysteine to generate \(\gamma\)-glutamyl-cysteinyl-glycine or GSH, and this second step is catalyzed by glutathione synthase 33