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Immunoblot analyses of ERK1/2 phosphorylation (p-ERK) revealed an ECM-dependent modulation of ERK activity, with FN-grown iMCs featuring highest p-ERK levels, and COL I-exposed MCs displaying lowest p-ERK levels (Fig

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References: (1) Wang, XingChun, et al

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Centrifuge at 600 gav for 15 min at room temperature (approx 20C)