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This area of research has been inspired by Otto Warburg's observations in 1922, wherein he discovered the specific glucose consumption by cancer cells through the Warburg metabolic pathway [2]

It was developed at Washington State University by the research group of Joseph Harding and John Wright, who were studying the cognitive effects of angiotensin IV and its analogs

A guide to ferroptosis in cancer

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After entering cells, DCFH-DA converts to DCFH and forms fluorescent dichlorofluorescein when oxidized by ROS