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Quantitative real-time polymerase chain reaction (qRTPCR) To determine mRNA expression, total RNA was extracted from each sample group using TRIzol reagent, and cDNA synthesis was performed using a Transcription First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland)

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[DOI] [PubMed] [Google Scholar] 142.Anwar S., Raut R., Kanwal B., Yahia E.A