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DN30 is involved in decreasing the c-Met signaling pathway through a variety of ways, including c-Met destruction, reducing c-Met expression, c-Met proteolytic detachment, and shedding c-Met from the cell surface, providing traps for HGF [162, 170]

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In brief, gene fragments were obtained from Integrated DNA Technologies or Twist Biosciences or amplified using Q5 polymerase (New England Biolabs, M0491S)