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To exclude potential kinetic differences in the ability of DA to recruit -arrestin2 versus G i1 , time-course experiments showed that receptor stimulation by DA does not result in the recruitment of -arrestin2 at any of the time points tested (data not shown)

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Consequently, we observed that this beneficial effect, after direct injury (permanent ligation) applied to one or two major vessels, could instantly oppose more general damage (maintained intra-abdominal hypertension, either high (grade III) or very high (grade IV)), as all blood vessels which can be compressed with increased intra-abdominal pressure