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Throughout the planning process, many opportunities for public input and discussion were facilitated

Studies have also shown that imbalances in gut microbiota can amplify the inflammatory response to LPS, a component of gram-negative bacteria, aggravating DED (Bertani and Ruiz, 2018

After an additional twenty-four hours, media was exchanged with fresh DMEM +dFBS / RPMI +pyruvate +dFBS /McCoys 5A +dFBS that was incubated overnight at 5% CO 2 supplemented with 300 ng/mL of Dox (for HeLa and Caki-1 lenti lines)

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