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The DNA was amplified by polymerase chain reaction (PCR), and then sequencing was done by capillary electrophoresis using Applied biosystem 3500 Genetic Analyzer (Waltham, MA, USA) with forward primer 5-AGACCTCACAGTAAAAATAGGTGA-3 and reverse primer 5- CTGATGGGACCCACTCCATC-3

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These remain essential for long-term success