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Published 2017 May 15.

Second, elevated lactate directly inhibits immune cell activation/proliferation and drives Kla-mediated immunosuppression, promoting CTLA-4 expression in T cells, M2-like macrophage polarization, and dendritic cell dysfunction (47)

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Antigen retrieval with 10 mM sodium citrate buffer (Merck Life Science N.V, Sigma, Netherlands #6132043) was done on OCT sections in a pressure cooker for 30 min