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We have previously shown HA14-1 to decrease the cellular GSH pool with a propensity to affect the mitochondrial GSH pool first and induce mitochondrial oxidative stress and intrinsic apoptosis in CGNs [37, 38]

After electrophoresis, the gel was stained with a staining solution [0.1 % coomassie brilliant blue (CBB) R250, 45 % methanol, and 10 % acetic acid] for 2 h

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